ip lab image analysis software  (scanalytics inc)

Journal: Scientific Reports

Article Title: Plin5 alleviates myocardial ischaemia/reperfusion injury by reducing oxidative stress through inhibiting the lipolysis of lipid droplets

doi: 10.1038/srep42574

Figure Lengend Snippet: ( A ) Electron microscopy images of mouse myocardium with or without I/R injury (n = 4). Mitochondrial sizes were analysed using Image pro plus 6.0 software in four electron microscopic images of the myocardium from wild-type and Plin5 -null mice. Scale bar = 1 μm. ( B ) Relative mitochondrial DNA (mtDNA) contents (normalised to the single-copy nuclear gene Ndufv1) in the myocardium of wild-type and Plin5 -null mice (n = 4). ( C ) Immunoblotting analysis of the levels of mitochondria markers in the myocardium of mice (n = 4). ( D ) Quantitative PCR analysis of the mRNA levels of mitochondria markers in the myocardium with or without I/R injury (n = 4). The columns and errors bars represent the means ± SEM. * P < 0.05, ** P < 0.01.

Article Snippet: The Evans blue-stained area (area not at risk, ANAR), the TTC stained area (red colour, ischaemic but viable tissue), and the TTC stained negative area (white colour, infarcted tissue) were digitally measured using an IP Lab Image Analysis Software (Version 3.6; Scanalytics).

Techniques: Electron Microscopy, Software, Western Blot, Real-time Polymerase Chain Reaction